Skip to main content
. Author manuscript; available in PMC: 2011 Oct 11.
Published in final edited form as: J Immunol. 2010 Mar 29;184(9):5085–5093. doi: 10.4049/jimmunol.0902710

FIGURE 5.

FIGURE 5

E. muris infection suppressed the IgG response to an irrelevant Ag. E. muris-infected mice and uninfected control C57BL/6 mice were immunized i.p. with NP-CGG in alum on the indicated days postinfection. To assess the NP-specific IgG response, serum and spleen tissue were harvested on day 12 postimmunization and were analyzed by ELISA (A) and ELISPOT analysis (B, C) in mice immunized with NP-CGG only (●) and in E. muris-infected, NP-CGG–immunized mice (■). The frequency (B) and number (C) of splenic NP-specific IgG-secreting cells was determined by ELISPOT analysis after subtracting background spots obtained using cells from mock-infected control mice. D, Day 9 E. muris-infected and mock-infected mice were immunized s.c. with NP-CGG in alum, and the NP-specific IgG response was analyzed at day 21 postinfection by ELISPOT analysis in the spleen (top row) and pooled brachial and axillary draining LNs (bottom row). The data are representative of three independent experiments in which three mice were analyzed at each time point. *p ≤ 0.05.

HHS Vulnerability Disclosure