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. 2011 May 27;286(34):29449–29461. doi: 10.1074/jbc.M111.257600

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Expression of mouse MsrB1 in Drosophila. A and B, selenoprotein pattern in fruit fly homogenates visualized using a PhosphorImager system. Expression of mouse MsrB1 in the whole body (A) or the nervous system (B) was obtained by crossing homozygous UAS-mMsrB1 flies with da-GAL4 activator line or elav-GAL4 activator line, respectively. Panels A and B have the same order of samples: lanes 1 and 2, cross GAL4-activator/mMsrB1^3A; lanes 3 and 4, cross GAL4-activator/mMsrB1^2A; lanes 5 and 6, cross GAL4-activator/mMsrB1^3B; lanes 7 and 8, control cross GAL4-activator/yw. Drosophila selenoproteins (dSPS2, 43 kDa; dSelH, 28 kDa; mMsrB1, 12.6 kDa; and dSelK, 11.5 kDa) are marked on the left side of panel A with arrows. Molecular mass markers are shown on the right side of panel A. C and D, Coomassie Brilliant Blue staining of membranes A and B, respectively (protein loading control). Genders are shown by symbols below panels C and D.