FIGURE 10.

Interferon β included as a co-stimulant during LPS pretreatment induces endotoxin tolerance in human gingival fibrobalsts after repeated challenge with both E. coli LPS (a) and P. gingivalis LPS (b) (concentrations of IFN-β are in ng/ml). HGFs (8 × 10⁴ cells/ml) were pretreated with 10 ng/ml E. coli LPS or 10 μg/ml P. gingivalis LPS with or without the addition of rhIFN-β for 24 h and re-treated with the same LPS for 4 h. IL-8 was measured in the cells supernatants. Values represent the mean ± S.E. of n = 3.