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IL-8 (a) and TNF-α (b) production by THP-1 cells cross-tolerized with E. coli (E.c) LPS and P. gingivalis (P.g) LPS. THP-1 cells were pretreated with 1 μg/ml E. coli LPS for 24 h and re-treated with 1 μg/ml P. gingivalis LPS for 4 h or vice versa. Control THP-1 cells were incubated in medium alone and re-treated with 1 μg/ml E. coli or P. gingivalis LPS. Supernatant was removed, and IL-8 and TNF-α levels were assessed by ELISA.
