FIGURE 6.

The addition of rIFN-β during P. gingivalis (P.g) LPS pretreatment delays IκB-α degradation after repeated challenge with P. gingivalis LPS. THP-1 cells were pretreated for 24 h with medium alone (a), 1 μg/ml P. gingivalis LPS (b), and a combination of 1 μg/ml P. gingivalis LPS and 0.1 ng/ml IFN-β (c). Cells were washed and re-treated with 1 μg/ml P. gingivalis LPS for the indicated time points. Whole cell lysates were immunoblotted for IκB-α and GAPDH as a loading control. Blots are representative of three separate experiments.