FIGURE 7.

The addition of rIFN-β during 24 h P. gingivalis (P.g) LPS pretreatment significantly decreases production of IL-8 by THP-1 cells after repeated exposure to P. gingivalis LPS. Neutralizing IFN-β antibody included during E. coli (E.c) LPS pretreatment recovered IL-8 production by THP-1 cells re-treated with E. coli LPS. Isotype control antibody did not have influence on establishment of endotoxin tolerance. rIFN-β alone is not able to induce endotoxin tolerance. IL-8 in cell supernatants was assessed by ELISA. Values represent the mean ± S.E. of n = 3 (endotoxin activity of rIFN-β < 0.1 ng/μg).