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IFN-β does not have effect on decreased production of TNF-α after repeated challenge with E. coli LPS. THP-1 cells were pretreated with medium alone, 1 μg/ml E. coli (E.c) LPS, 1 μg/ml E. coli LPS + 1 μg/ml anti-IFNβ antibody, and 1 μg/ml E. coli LPS + 1 μg/ml isotype control antibody and re-treated with 1 μg/ml E. coli LPS. TNF-α was measured in the cell supernatants by ELISA. Values represent the mean ± S.E. of n = 3.
