FIGURE 5.

Egr1 siRNA blunts the insulin-dependent induction of cholesterol biosynthetic genes. H4IIE cells were transfected with either control siRNA or Egr1-specific siRNAs. Data are displayed relative to gene inductions in control transfected cells. RT-qPCR indicated a 35% reduction in the response of Hmgcr to insulin in cells transfected with siRNA for Egr1 after treatment with insulin for 1.5 h. Similar reductions were observed for other cholesterol biosynthetic genes such as Me1 and Sqle. Average values from four independent experiments are shown, and error bars represent S.D. Asterisks indicate p < 0.05 (Welch's t test).