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. 2011 Jun 30;286(34):29511–29520. doi: 10.1074/jbc.M111.236810

FIGURE 4.

FIGURE 4.

Cell contraction induced by ARHGEF10 T332I in HEK293T cells. A, domain structures of ARHGEF10. Full-length ARHGEF10 (wt) and the T332I mutant of full-length ARHGEF10 are shown. B, HEK293T cells transiently transfected with the plasmid encoding GFP, GFP-ARHGEF10 wt, or GFP-ARHGEF10 T332I. After 48 h, fluorescence images of living cells were observed. Scale bar, 10 μm. C, quantitative analysis of cell contraction of HEK293T cells. HEK293T cells were transiently transfected with the plasmid encoding GFP, GFP-ARHGEF10 wt, or T332I. The proportion of cell contraction was scored as a percentage of the rounded cells of GFP-positive cells. Cells floating in the culture medium were excluded from counting. The data represent the mean ± S.E. (error bars) from three independent experiments. *, p < 0.05 compared with empty. For each experiment, >100 cells were counted. D, HEK293T cells transiently transfected with the plasmid encoding GFP-ARHGEF10 wt or T332I. After 48 h, cells were fixed and co-stained with TRITC-phalloidin (F-actin) and Hoechst33258 (DNA). Transfected cells are shown by the fluorescence of GFP. Scale bar, 10 μm.