FIGURE 1.

Effects of MS-275 on HepG2 cells. A, HepG2 cells were treated with MS-275 for 24 h, and cell lysates were subjected to Western blot analysis using anti-acetylated histone H3 and H4 antibodies (Ac-H3 and Ac-H4). Total histones H3, H4, and actin were used as loading controls. B, cells were treated with MS-275 for 24 h (triangles) or 48 h (squares). Cell counts are expressed as viable cells per well. C, DNA synthesis was assessed by thymidine incorporation in the presence of the indicated concentrations of MS-275. D, cells were treated with (squares) or without (triangles) 6 μm MS-275 for 24 h, and then floating and adherent cells were collected, pooled, and assayed for caspase-3 activity over 160 min. Mean data are expressed ± S.D. of triplicate determinations, and results are shown from one representative experiment of three.