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. 2011 Jul 1;286(34):29644–29653. doi: 10.1074/jbc.M111.268094

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Mef2A knockdown in neonatal cardiomyocytes decreases cell viability. A, upper panels, representative DAPI-stained images from each knockdown culture: shLacZ (left panel) and shM2A (right panel). Lower panel, quantification of nuclei in transduced NRVMs. DAPI-positive nuclei counted from 10 distinct areas (10× magnification) in transduced cultures from two experiments revealed a 44% reduction in nuclei number in the shM2A cultures (#, p ≤ 0.008). B, CellTiter-Blue assay of cell viability. Cell survival is significantly decreased in shM2A cultures (*, p ≤ 0.05). C, Western blot analysis of cleaved caspase-3 expression showed an increase in caspase-3 in shM2A cultures. GAPDH is shown as a loading control. D, qRT-PCR expression analysis of BIM in shM2A transduced NRVMs. BIM expression is significantly stimulated 2.3-fold (*, p ≤ 0.012) in shM2A cultures.