FIGURE 4.

ATP13A2 protects neurons from manganese-induced apoptosis. A, rat hippocampal neurons were cotransfected with GFP and ATP13A2WT or the ATP13A2DUP22 mutant and treated with 0 and 800 nm MnCl₂ for 24 h. Representative images of DAPI staining are shown. Apoptotic cells with nuclear condensation and fragmentation in GFP-positive cells are indicated (red arrows). Scale bar = 10 μm. B, the results from quantification of three independent experiments are show. Results are presented as means ± S.D.