FIGURE 6.

Myr-SKAP1 overrides the need for PI3K in the regulation of LFA-1 adhesion. T8.1 cells were transfected with SKAP1-WT, myr-SKAP1 WT, SKAP1-R131M, or myr-SKAP1-R131M; ligated with anti-CD3 in the presence or absence of wortmannin; followed by an assessment of membrane binding by blotting (A) or binding to ICAM-1 on plates as described under “Experimental Procedures” (B). Lysates were blotted with anti-GFP and anti-β-actin (A, lanes 1–8). Error bars represent S.D. For paired t tests, experimental groups were compared with their respective groups. Significant differences (p ≤ 0.05) are indicated with asterisks.