FIGURE 1.

Expression and purity of AtClpC2 and AtClpD. Expression and purification of the recombinant proteins were monitored by gel electrophoresis. The content of each lane is identical for AtClpC2 (A) or AtClpD (B). Soluble extracts from uninduced and induced cultures were loaded in lanes 1 and 2, respectively. Lane 3 shows the eluted proteins after affinity chromatography and lane 4, the purified proteins after the whole purification procedure. Molecular weight standards are depicted on the left. C, purified AtClpD was transferred to two nitrocellulose sheets and detected by antibodies raised against the polyhistidine tag (a-His) or against AtClpD (a-AtClpD). D, purified proteins were blotted after electrophoresis and detected with anti-ClpC (left) or anti-AtClpD (right) antibodies. C2 and D indicate lane content (AtClpC2 and AtClpD, respectively).