FIGURE 4.

Profile for reactivation of soman-inhibited Y337A/F338A hAChE upon 10 min of incubation with the library of 840 oximes (0.67 mm in 0.1 m phosphate buffer, pH 7.4, at 37 °C). Reactivated hAChE activity was measured by continuous activity assay (ΔA/min) at 412 nm and compared with HI-6 and 2-PAM recovered activity indicated by separate white and gray bars. Percent of hAChE inhibition by 6.7 μm oximes seen in the activity assay in the presence of 1.0 mm ATCh is also shown (light gray bars in the background).