TABLE 2.

Comparison of second order reactivation rate constants (k_r), maximal reactivation rate constants (k_max), Michaelis-type constants (K_ox), and the final amount of recovered activity (percent of uninhibited control) for reactivation of soman-inhibited hAChE mutants, with HI-6 in 0.1 m phosphate buffer, pH 7.4, at 22 °C

hAChE-mutant	Kox	kmax	kr	Recovery
	μm	min−1	m−1min−1	%
F338A	81 ± 34	0.027 ± 0.002	330 ± 130	19–64
F295L/F338A	45 ± 18	0.024 ± 0.002	520 ± 180	9–33
E202Q/Y337A	28 ± 11	0.057 ± 0.004	2000 ± 650	40–81
Y337A/F338A	400 ± 99	1.0 ± 0.1	2500 ± 520	28–70
Y337A/F338Aa	550 ± 290	1.1 ± 0.3	2100 ± 1200	65–95

^a Inhibited enzyme was prepared using soman nerve agent instead of the soman fluorescent-MP analog O-pinacolyl O-(3-cyano-4-methyl-7-coumarin) methyl phosphonate.