TABLE 2.
hAChE-mutant | Kox | kmax | kr | Recovery |
---|---|---|---|---|
μm | min−1 | m−1min−1 | % | |
F338A | 81 ± 34 | 0.027 ± 0.002 | 330 ± 130 | 19–64 |
F295L/F338A | 45 ± 18 | 0.024 ± 0.002 | 520 ± 180 | 9–33 |
E202Q/Y337A | 28 ± 11 | 0.057 ± 0.004 | 2000 ± 650 | 40–81 |
Y337A/F338A | 400 ± 99 | 1.0 ± 0.1 | 2500 ± 520 | 28–70 |
Y337A/F338Aa | 550 ± 290 | 1.1 ± 0.3 | 2100 ± 1200 | 65–95 |
a Inhibited enzyme was prepared using soman nerve agent instead of the soman fluorescent-MP analog O-pinacolyl O-(3-cyano-4-methyl-7-coumarin) methyl phosphonate.