FIGURE 8.
IQGAP1 is overexpressed in trastuzumab-resistant SkBR3 cells. A, equal amounts of protein from trastuzumab-sensitive (SkBR3) and trastuzumab-resistant (SkBR3TR) SkBR3 cells were resolved by SDS-PAGE, transferred to PVDF membranes, and probed with anti-IQGAP1 and anti-β-tubulin antibodies. The data are representative of three independent experiments. B, the amount of IQGAP1 was quantified by densitometry and corrected for the amount of β-tubulin in the corresponding lysate. The data, expressed relative to the amount of IQGAP1 in SkBR3 cells, represent the mean ± S.E. (n = 3). *, significantly different from SkBR3 cells (p < 0.05).