TABLE 2.
Constructs used in this study
| Construct | Vector | Residues | Source |
|---|---|---|---|
| E. coli BbpN2N3 | pQE30 | 270–599 | This study |
| E. coli SdrGN2N3 | pQE30 | 273–597 | (3) |
| E. coli ClfAN2N3 | pQE30 | 229–545 | (27) |
| E. coli Fg Aα | pQE30 | Full-length mature Aα | (18) |
| E. coli Fg Aα1–575 | pQE30 | 1–575 of mature Aα | This study |
| E. coli Fg Aα1–560 | pQE30 | 1–560 of mature Aα | This study |
| E. coli Fg Bβ | pQE30 | Full-length mature Bβ | (19) |
| E. coli Fg γ | pQE30 | Full-length mature γ | (20) |
| L. lactis-vector | pKS80 | Empty vector | (23) |
| L. lactis-Bbp | pKS80 | Full-length Bbp | This study |
| S. aureus Newman DU6023-pCU1 | pCU1 | Empty vector | (23) |
| S. aureus Newman DU6023-pCU1-Bbp | pCU1 | Full-length Bbp (with promoter and terminator) | This study |