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AUC analysis of ME0052 binding to ypTpx. A line graph shows the binding of ME0052 to purified ypTpx during AUC experiments. The lines represent different redox states for Tpx: oxidized (black diamonds), reduced (blue inverted triangles) and forced reduced mutant, C61S (green triangles). The amount of bound ME0052 was used to calculate the Kd values described in the text.
