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Steroidogenic response to gonadotropin stimulation in WT and GRTH KO mice. A, testosterone response (serum and Leydig cell) to an in vivo single dose of hCG (0.5 μg, sc, 24 h) in WT and GRTH KO mice. B, testosterone response to in vitro hCG (100 ng, 3 h) stimulation in purified Leydig cells prepared from WT and KO mice. Data are presented as the mean ± S.E. of three independent experiments performed in triplicate. *, p < 0.05.
