FIGURE 2.

Abnormal mitochondrial morphology in Leydig cells of GRTH KO mice associated with high cholesterol content in the inner mitochondrial membrane. A, EM analysis of Leydig cells in WT and GRTH KO mice. Small arrows, mitochondria with normal morphology; large arrows, swollen mitochondria without normal central cristae. N, nuclear. Scale bars = 1 μm. B, cholesterol content in the inner mitochondrial membrane (Mito. Mem.) of purified Leydig cells from WT and KO mice. Animals were treated with a single dose of hCG (0.5 μg, sc, 24 h). Data are presented as the mean ± S.E. of three independent experiments performed in triplicate. *, p < 0.05.