FIGURE 7.

Coimmunoprecipitation of FLAG-MAGE-11 with human AR, mouse AR, human PR-B, and human GR. COS cells were transfected with 3 μg of FLAG empty vector or FLAG-MAGE and 1 μg of pCMV-hAR or 2 μg of pCMV-mAR in cells incubated with and without 10 nm DHT (A), 2 μg of pCMV-hGR in cells incubated with and without 10 nm dexamethasone (DEX; B), and 2 μg of p5M-hPR-B in cells incubated with and without 10 nm progesterone (PROG; C), all in the presence of 0.1 μm EGF. Cell extracts (50 μg of protein/lane) and immunoprecipitated proteins (IP) were analyzed by immunoblot using AR, GR, and PR antibodies.