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. 2011 Jul 7;286(34):29984–29992. doi: 10.1074/jbc.M111.249011

FIGURE 3.

FIGURE 3.

A, stereo diagram of the binding site for NADH in Pos5-NADH-A. NADH in Pos5-NADH-A is indicated in deep blue. Asp-256*, Cys-291*, and Arg-293* come from another subunit (Pos5-NADH-B′). B, stereo diagram of the binding site for NADH in Pos5-NADH-A as in Fig. 3A, the binding site for NAD+ in Ppnk-NAD+ (salmon pink; Protein Data Bank code 1u0r, A chain), and the corresponding region of human NADK (lime green; 3pfn, A chain). The residues denoted by asterisks come from another subunit. C, electric charge on the molecular surfaces of NAD(H)-binding sites of NADKs [human NADK (Protein Data Bank code 3pfn, A chain) and Ppnk-NAD+ (Protein Data Bank code 1y3h, B chain)] and NADHK (Pos5). Positive and negative charges at pH 8.0 are colored in blue and red, respectively. NAD+ and NADH are colored as in Fig. 1A. Residues are colored in blue (His, Lys, and Arg), red (Asp and Glu), and yellow (Cys, Asn, and Tyr). The residues denoted by asterisks come from another subunit in each structure.