FIGURE 4.

Expression of GFP-fascin mutants in fascin knockdown B16-F1 cells. A, B16-F1 cells co-expressing fascin-shRNA and GFP-fascin constructs (green). Cells are co-stained with phalloidin (red) to visualize F-actin. The expressed GFP-fascin constructs are indicated at the top of each column (mutants are named according to Figs. 2 and 3). Boxed regions are enlarged at the bottom (scale bar, 5 μm). B, quantitative analysis of the number of filopodia per cell based on phalloidin staining (red) or the GFP signal (green) for each GFP fusion construct. Data are shown as box-and-whisker plots: box length represents values from low to upper quartile, whiskers encompass 5th to 95th percentile, boxes are divided by the median, and a bar represents the mean value (n = 27–36 per construct).