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. 2011 Jun 28;286(34):30119–30129. doi: 10.1074/jbc.M111.258814

FIGURE 2.

FIGURE 2.

TGF-β1-induced miR143/145 expression in HCASM. A, heat map illustrating relative expression of a sample of miRs in triplicate control or TGF-β1 (1 ng/ml)-treated HCASM. B, qPCR validation of indicated miRs in HCASM treated with 1 ng/ml TGF-β1 for 24 h. The expression level of each miR reflects the -fold change relative to vehicle control (set to 1). C and D, qPCR analysis of miR145 (C) and two SMC contractile genes (D) in HCASM following 24-h treatment with varying concentrations of TGF-β1. E, HCASM were treated with TGF-β1 (1 ng/ml) for the indicated times, and qPCR was performed to measure normalized expression of miR143/145, miR21, and miR221. Relative miR expression is the -fold change relative to control (time 0) set to 1. All data shown are representative of at least three independent experiments. Error bars, S.D.