FIGURE 8.

miR145 contributes to TGF-β1-induced HCASM differentiation. A, growing HCASM were transfected with the indicated pre-miRs or a control miR mimic (30 nm each) for 72 h, and then qPCR was done for miR145 expression. B, Western blotting of the indicated SMC marker proteins in growing HCASM transfected as in A. Note the near absence of SMC markers in the control and miR21-transfected cells. C and D, TGF-β1 (1 ng/ml)-stimulated HCASM following transfection with 30 nm anti-miR to either control (open bar) or miR145 (filled bar) were processed for qPCR of miR145 (C) or Western blotting of the indicated SMC differentiation proteins (D). Note that serum starvation does not induce expression of SMC contractile proteins or miR143/145 (data not shown). Each experiment was replicated in at least two independent studies. Error bars, S.D.