FIGURE 8.

SiRNAs targeting PSK1-α/β or PSK2 inhibit mitotic cell rounding and spindle centralization. The percentages of siRNA-transfected mitotic cells that showed nonrounded and irregular morphology (A) or decentralized spindles (B) are shown. C, decentralization index shows the distance between each spindle pole and the closest cell membrane as a ratio of the longest length over the shortest length. Mitotic cells were imaged and scored as described under “Experimental Procedures.” Graphs and error bars show mean ± S.E. (n = 3). Analysis of variance (ANOVA) was performed to assess the significance of the results shown in the graph. Subsequently, a Dunnet's multiple comparison test was used to determine whether the differences were significant between cell populations transfected with control siRNAs or the PSK siRNAs indicated in the figure. *, p < 0.05; **, p < 0.01; ***, p < 0.001.