Fig. 3.

APOD promoter is responsive to signals from endothelial cells. A: diagram of APOD promoter depicting the region that was inserted upstream of the luciferase gene in pGL3-basic plasmid. B: luciferase reporter assays were performed to assess APOD promoter responsiveness to coculture and conditioned media. HDFNs were transiently transfected with the pGL3 empty vector or ApoD-921-pGL3-luciferase plasmid and cultured in the presence or absence of HUVECs, or supplemented with HUVEC-conditioned media. After 48 h, transcriptional activity was determined by luciferase activity. *P < 0.05.