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. 2011 May 27;301(3):H832–H840. doi: 10.1152/ajpheart.00249.2011

Fig. 1.

Fig. 1.

Phosphorylation changes in response to pulmonary hypertension. Shown is a summary of protein phosphorylation in control (CO) and high-altitude hypertensive animals (HA). A: 12.5% SDS-PAGE gel images representative of five control and five HA animals. B: phosphosignals [Pro-Q diamond gel stain (PQD)] for myosin-binding protein C (MyBP-C), desmin, troponin T (TnT), (total) troponin I (TnI), and myosin light chain (MLC)2 were normalized to total MLC1 protein expression to correct for loading differences. TnI p22/23 phosphorylation was measured using a phosphospecific antibody and was normalized to the total TnI signal after reprobing the same membrane with a pan-specific TnI antibody. n = 5 control and 7 HA. RV, right ventricle. *P < 0.05. Molecular weight standards are in kiloDaltons.