Figure 4.

ABI5 protein accumulation is induced by salt and drought stress. (A) WT Ws seeds were kept in darkness at 4°C for 3 days with or without NaCl (200 mM) and then transferred to constant light at 22°C for 1, 2, 3, 4, and 5 days (0 indicates the time immediately following transfer). Western blot analyses using antibodies to ABI5 were performed as described in Fig. 1. (B) One-day-old seeds were transferred to absorbing paper for 1, 2, 3, and 4 h. Western blot analyses using antibodies to ABI5 were further performed as described in Fig. 1.