Fig. 4.

In vitro protein expression of p-p38 and p-JNK MAPK. A and B: H₂O₂ (10 μM/15 min) increased p-p38 expression 3.1 ± 1.4-fold vs. control (*P < 0.05; n = 5) but was unaffected by APN pretreatment. C and D: additionally, neither H₂O₂ nor APN pretreatment had any effect on p-JNK protein expression (n = 5). p-p38 and p-JNK are normalized to GAPDH protein expression for equal loading. Results are expressed relative to control.