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In vivo NAD(P)H oxidase expression. A: ANG II infusion (3.2 30 mg·kg−1·day−1 for 14 days) increased expression of NAD(P)H oxidase subunit p22phox by 20 ± 2% in WT mouse LV (†P < 0.001 vs. WT saline). p22phox expression was further enhanced by an additional 38 ± 6% in APN-KO (#P < 0.01 vs. WT ANG II). B: similarly, ANG II infusion increased p47phox expression by 31 ± 5% in WT mouse LV (#P < 0.01 vs. WT saline) and was further increased by 28 ± 0.5% in APN-KO (#P < 0.01 vs. WT ANG II).
