Table 1. Primers used for CCHFV genetic characterization of the S and M segments.
Segment S | ||
Fragment 1 (590 bp) | ||
1st round | CCHF-MU: TCTCAAAGATATCGTTGCC | CCHF-ER7: GAATTAGGGAAGCAACCAAG |
Fragment 2 (550 bp) | ||
1st round | CCHF-F2b: AAAGAGATGTTGTCAGACATGAT | CCHF-R2b: GTTTCTTTCCCCACTTCATTGG |
2nd round | CCHF-F3b: GAAGAAGGAACTTGATCCTCAA | CCHF-R2b |
Fragment 3 (536 bp) [19] | ||
1st round | CCHF-F2: TGGACACCTTCACAAACTC | CCHF-R3: GACAAATTCCCTGCACCA |
Segment M | ||
Fragment 1 (280 bp) | ||
1st round | CCHF-M1F: AATGCAATAGATGCTGAAATGCA | CCHF-M2R: TTGYTTGCYTC1AYRGTYGC |
2nd round | CCHF-M1F | CCHF-M1R: GAYTGRACTGG1GAYAWYGAAAC |
Fragment 2 (420 bp) | ||
1st round | CCHF-M2F: CAAGTRTCRGAGTCAACRGG | CCHF-M2R: TTGYTTGCYTC1AYRGTYGC |
2nd round | CCHF-M3F: TGGCTCTRAAGAGRAGYTGYTGGATRA | CCHF-M3R: TTRCARACRGCYAGCATRACATT |
Fragment 3 (510 bp) | ||
1st round | CCHF-M4F: GAGTC1CAYAAATGCTAYTGYAGTCT | CCHF-M4R: ACTGAACTCCAGCTAAGTGCTA |
2nd round | CCHF-M4F | CCHF-M5R: GTTGAYTGRACATTRATTGCYCCCCA |
Sequences are reported in 5′-3′ orientation.