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. 2011 Oct 11;6(10):e25707. doi: 10.1371/journal.pone.0025707

Figure 1. Full length hGSTP1 directs expression of catalytically-active GSTπ.

Figure 1

When transfected into Hep3B cells, which do not express hGSTP1 mRNA or hGSTP1 polypeptides, (A) both full length hGSTP1 (−1138 to +3600) and hGSTP1 with [ATAAA]n ([AT]n) repeat sequences deleted (−78 to +3600) directed expression of (B) GSTP1 polypeptides, as detected by immunoblot analysis, and (C) active GSTπ, as detected by CDNB assay using the indicated volumes of HEP3B cell lysates, with appearance of the glutathione conjugate of CDNB monitored as change in OD340 nm. (A–C) Yellow box, position of [AT]n repeat; Green box, position of CpG island; positions are shown with respect to the hGSTP1 transcriptional start site; blue boxes, position of exons; rhGSTP1, recombinant human GSTP1 polypeptides in vitro. Transfection controls directed expression of GFP polypeptides.