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. 2011 Oct 11;6(10):e25705. doi: 10.1371/journal.pone.0025705

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Young micropropagated olive plants were inoculated with P. savastanoi pv. savastanoi NCPPB 3335 (wild type) and Psv48ΔA (cured of pPsv48A). (A) Light microscopy images of semithin cross sections of knots (35 dpi) stained with toluidine (B, C) Cross-sections of knots, collected at 28 dpi, stained with methylene blue-picrofuchsin. Asterisks indicate the position of newly formed xylem vessels. (B) Parenchymatous-like cells showing a blue-purple stain of the cell walls (wild type) due to the formation of secondary walls during differentiation. (C) Detail of newly formed bundles of xylem vessels.