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. 2011 Oct 11;6(10):e25419. doi: 10.1371/journal.pone.0025419

Figure 2. IFN-α restores the expression level of PCBP2 in R1b cells after inhibiting the expression of HCV replicon.

Figure 2

(A) Western blotting analysis indicated prominent up-regulation of PCBP2 in R1b cells after treated with 100 IU/mL IFN-α, while no significant differences were noted in Huh7.5.1 cells. (B) The restoration of the expression of PCBP2 is IFN-α dosage dependent and due to inhibition of HCV NS proteins. IFN-α doses of 50, 100 and 200 IU/mL were used to treat R1b cells for 6 hours. Western blotting was performed to detect the expression of proteins. (C) PCBP2 protein level in other hepatocyte-derived cells after treatment of 100 IU/mL IFN-α. The PCBP2 level was unchanged, similar to that observed in Huh7.5.1 cells. (D) The protein level of PCBP2 was restored in cured R1b cells. The PCBP2 level did not return to a low level when IFN-α was removed from the media for a week.