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. 2011 Oct 11;6(10):e26179. doi: 10.1371/journal.pone.0026179

Figure 3. Excision of the MccH47 genetic system.

Figure 3

A. Schematic representation of the PCR approach performed to detect the excision of the MccH47 system. Amplification products containing the attC and attI were obtained with primer pairs out1-out2 and in1-in2, respectively. B. Detection of the MccH47 system excision from plasmids pEX2000 and pEX100 with the indicated primer pairs in the context of RYC1000 cells. L: 1,5 kb DNA ladder (Bioron). C. Detection of the MccH47 system excision from the E. coli H47 chromosome. L: 100 bp DNA ladder (Bioron).