Figure 3.

Analysis of JMT gene expression by RT-PCR. Two micrograms of total RNA isolated was amplified by RT-PCR, and the products were analyzed by using a JMT gene-specific probe. (A) Organ- and developmental stage-specific expression. (B) Stage-specific expression during the flower development. Flower stages are designated according to Bowman (28). Stage 13 is at the anthesis. (C) Local expression induced by various treatments. Expression of PDF1.2 and JR2 genes are also analyzed as references. (D) Systemic expression induced by wounding or MeJA. The treated leaves (local) were enclosed in a plastic bag to be separated from distal leaves (systemic) and the plants were kept in an open area.