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Kinetic parameters of JMT
| Substrate | Km, μM | Vm, nmol/ min | kcat, s−1 | kcat/Km, μM−1⋅s−1 |
|---|---|---|---|---|
| S-adenosyl methionine | 6.3 ± 0.7 | 84 ± 8.2 | 70 ± 6.8 | 11.1 ± 0.9 |
| (±) Jasmonic acid | 38.5 ± 5.8 | 30 ± 3.6 | 25 ± 3.0 | 0.7 ± 0.05 |
The values are the average of three independent measurements. Each value was obtained by Lineweaver-Burk plots, which were linear within experimental error.
