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. Author manuscript; available in PMC: 2012 Oct 19.
Published in final edited form as: J Proteomics. 2011 Apr 15;74(11):2510–2521. doi: 10.1016/j.jprot.2011.04.007

Figure 1. Overview of experimental approach to generate, indentify, and validate 3NT peptides.

Figure 1

An equimolar mixture of 48 proteins, UPS1, was TNM or mock treated to generate a set of nitrated and control samples. Samples were trypsin digested and analyzed with by both QSTAR Elite (QTOF) and LTQ Velos (dual ion trap) LC-MS/MS systems. Data was searched with Mascot and the each resulting 3NT peptide was analyzed by manual inspection using the criteria defined in Table 1.