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Workup for Clostridium difficile PCR ribotype 002 in a healthcare region in Hong Kong (1 January 2009 to 31 December 2009). a169 strains from 145 patients were found to have cytotoxin production by cell culture cytotoxicity neutralization assay (CCCNA) from both stool filtrates and C. difficile isolates. Another 176 strains from 162 patients had negative cytotoxin production in stool filtrates but positive toxin production in the culture supernatant of C. difficile isolates
