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. 2001 Mar 27;98(8):4794–4799. doi: 10.1073/pnas.071041998

Figure 2.

Figure 2

Analysis of peanut seed proteins after reduction by the NADP/thioredoxin system or DTT and labeling with mBBr using nonreducing/reducing two-dimensional SDS/gel electrophoresis. Fifty micrograms of peanut protein (20 μl extract) was incubated in 50 mM Tris⋅HCl buffer, pH 7.9, in a final volume of 100 μl. (A) Control: no addition. (B) DTT + heat: the sample was heated in boiling water after addition of 5 mM DTT. (C) Thioredoxin (Trx) (3 h): incubation for 3 h at 37°C in the presence of 0.125 μmol NADPH, 2.4 μg C. reinhardtii thioredoxin h and 2.1 μg A. thaliana NTR. (D) Thioredoxin (Trx) (5 h): incubation under the same conditions as C, except for 5 h.