Figure 3.

Analysis of peanut seed proteins after reduction by the NADP/thioredoxin system and labeling with mBBr using isoelectric focusing/reducing SDS/PAGE/mBBr. One hundred fifty micrograms of peanut protein (60 μl extract) was incubated in 50 mM Tris⋅HCl buffer, pH 7.9, in a final volume of 300 μl. (A) Control: no addition. (B) Thioredoxin (Trx) (3 h): incubation for 3 h in the presence of 0.375 μmol NADPH, 7.2 μg C. reinhardtii thioredoxin h, 6.3 μg A. thaliana NTR. IEF, isoelectric focusing.