Table 1.
Internal amino acid sequence analysis of thioredoxin target and nontarget peanut proteins
| No. | Internal sequence | Homologous protein | Amino acid matches |
|---|---|---|---|
| Nonreducing/reducing two-dimensional SDS/PAGE target protein (Fig. 2 no.) | |||
| 2 | QQWELQGDR | Peanut 2S protein (allergen Ara h2, oxidized) | 9/9 |
| 3 | ANLRPCcmEQHLMQ | Peanut 2S protein (allergen Ara h2, partially reduced) | 12/12 |
| NLPQQCcmGLR | 9/9 | ||
| 6 | RQQWEL | Peanut 2S protein (allergen Ara h2, fully reduced) | 6/6 |
| CmBBrCmBBrNELNEFENN | 11/11 | ||
| NLPQQCmBBrGLR | 9/9 | ||
| 5 | SQSENFEYVAFK | Peanut glycinin (allergen Ara h3 basic subunit) | 12/12 |
| 4a | ALGSVLAGDKDSLAYGR | Desiccation-related protein from resurrection plant | 11/17 |
| KLVAGLLGVESGQDAVIR | 15/18 | ||
| NEQVHPYGVSVATFTNR | 9/17 | ||
| ANLDAFTR | 5/8 | ||
| ISILR | 4/5 | ||
| LTXYVGTNPELQNP | 9/13 | ||
| DVILQFAYQEVGHLR | 11/15 | ||
| 4b | IAQFGSDVPMK | Seed maturation protein from soybean | 11/13 |
| AIAADLGYDENCmBBrK | 8/11 | ||
| IAVITGGDSGIGR | 12/13 | ||
| Nontarget proteins (Fig. 2 no.) | |||
| 7 | VQIEAKPNTLVLPK | Peanut vicilin (allergen Ara h1) | 14/14 |
| 8 | GYFGLIFPGCcmPSTYEEP | Peanut glycinin (allergen Ara h4 acidic subunit) | 17/17 |
| 9 | VYDEELQEGHVLVVPQN | Peanut glycinin (allergen Ara h4 basic subunit) | 17/17 |
| 10 | WLGLSAEYGNLYR | Peanut glycinin (allergen Ara h4 basic subunit) | 13/13 |
| 1 | XLSPDRK | Peanut glycinin (Gly1) | 6/6 |
| XFNLAGNHEQEFLR | 13/13 | ||
| XENESEEQGAIV | 12/12 | ||
| SPDIYNPQAGSLK | 13/13 | ||
| TANDLNLLILR | 11/11 | ||
| Isoelectric focusing/SDS/two-dimensional PAGE (Fig. 3 no.) | |||
| 31 | GKEEHKPIVVDF | Thioredoxin h from Chlamydomonas | 12/12 |
| 30 | XMGEQEQYDSYDIR | Peanut 2S protein (allergen Ara h6 isoform) | 10/13 |