Figure 2. Scutellaria baicalensis (SB) enhances IRF-1 activity.
A, IRF-1-luc/AGS cells were treated with indicated concentrations of SB extract. Luciferase activity was determined after 30h as described in Methods. B, IRF-1-luc/MDA468 cells were treated with SB extract for 30h, after which luciferase activity was measured. C, AGS cells were treated with SB extract for 24h or with 20μg/ml SB extract for the times indicated. Cell lysates were immunoblotted for IRF-1, p21, and β-actin. Densitometry ratios of indicated protein over β-actin, then normalized to 0 treatment are listed below each band as appropriate.