Table 1c. Additional Confirmation of Candidates.

To test the MUC16 selectivity of the nominated compounds following the A2780 isogenic screens additional matched, isogenic MUC16 positive/negative pairs were examined. The IC50’s, obtained graphically following 96 well plate Alamar Blue assays are shown. While 3 of 4 compounds were confirmed as more selective for MUC16 expressing cells in the SKOV3 pair, 4 of four were confirmed in the T80 paired cell lines and 3 of 4 (except Quinacrine) were more toxic for the MUC16 expressing SKOv8 cells than for the stable siRNA MUC16 knockdowns which show less than 10% of the parental levels of MUC16.

Compounds	SKOV3		T80		SKOV8		CAOV3	OVCAR3
	No MUC16	MUC16+ve	No MUC16	MUC16 +ve	No MUC16	MUC16 +ve	MUC16 +ve	MUC16 +ve
					Knock Down	Scramble	WT	WT
Ellipticine	4.5	4	0.3	0.25	0.2	0.11	0.6	0.7
Quinacrine dihydrochloride dihydrate	4.125	3.5	4.5	3.5	0.5	1.1	8	7
Acriflavinium hydrochloride	4.25	5.5	1.8	1.4	0.5	0.25	0.7	1
Rutilantinone	50	47.5	10.8	9.5	19	15	9	10