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The systemic injection of ddC increased TNFα in the rat lumbar DRG sections. Animals received a single ddC injection and tissue was collected at 14 days after ddC injection. The systemic injection of ddC induced clear immunoreactivity of TNFα compared to the saline group. Bar graph showed that systemic injection of ddC significantly increased TNFα in the DRG using a Western blot, compared to the saline group, *p < 0.05 vs saline, t test. n=3–6.
