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Expression and subcellular localization of Six3OS and Six3 in the developing retina. (A-H) Analysis of adjacent sections using chromogenic ISH for Six3OS and Six3 indicates substantial overlap of Six3OS and Six3 expression at E16.5 (A-D) and P0.5 (E-H). (I, J) Section fluorescent in situ hybridization (FISH) showing Six3OS expression. At E14.5, Six3OS is localized in both the nucleus (crosshairs) and the cytoplasm (I).
