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. 2011 Sep 29;5:152. doi: 10.1186/1752-0509-5-152

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Copyright ©2011 Zhang et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Positive regulation of pou6f1 to tmem59 is evident in C17.2 NSCs. (A, B) gray photos were captured in white light. (C, D)POU6F1-EGFP (green) was over-expressed in the nucleus of C17.2 neural stem cells. Photography was captured at 36-hours after transfection of pEGFP-N2-Pou6f1 plasmid. (E) Real-time PCR showed that tmem59 is up-regulated by over-expression of pou6f1 (normalized to β-actin). N = 3, **P < 0.01. OE: over-expression of POU6F1-EGFP; Con: control group transfected with pEGFP-N2.